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The Biogenesis of Vacuoles: Insights from Microscopy
1997
Publisher Summary Vacuoles of plant cells were discovered with the early light microscope and, as meant by the etymology of the word, were subsequently defined as the cell space empty of cytoplasmic matter. As usual in experimental science, methodological and instrumental progress has delineated differently the present definitions of vacuoles, which largely depend on the tools and concepts used for their study. This chapter discusses the biogenesis of vacuoles. Biochemistry and physiology of the vacuole have been made possible by the isolation of intact vacuoles. A specific, although yet incomplete, set of soluble and membrane proteins with unique properties have been specifically ascribed …